The oxidase test is a rapid biochemical assay used to determine whether bacteria produce cytochrome c oxidase, an enzyme involved in the electron transport chain. This test is commonly used to distinguish between oxidase-positive organisms (e.g., Pseudomonas, Neisseria, Vibrio) and oxidase-negative organisms (e.g., Enterobacteriaceae such as Escherichia coli).
✅ Rapid and Reliable – Provides results within 30 seconds, allowing for quick bacterial identification.
✅ Easy to Use – Requires minimal training and can be performed with a simple reagent application.
✅ High Sensitivity – Effectively detects cytochrome c oxidase activity, ensuring accurate differentiation.
✅ Versatile Applications – Suitable for clinical, environmental, food safety, and research laboratories.
✅ Stable and Convenient – Long shelf life and ready-to-use format make it an ideal choice for microbiologists.
Obtain a bacterial culture – Use a fresh colony grown on a non-selective medium.
Apply TestOxidase™ reagent – Place a drop of reagent onto a filter paper or directly onto the bacterial colony.
Observe the reaction – A dark purple color within 30 seconds indicates a positive result, while no color change signifies a negative result.
🔬 Clinical Diagnostics – Aids in identifying bacterial pathogens in medical laboratories.
🌿 Environmental Microbiology – Used to study bacterial diversity in various ecosystems.
🥗 Food Safety Testing – Helps detect contaminants in food and water sources.
🧪 Research Laboratories – Supports studies on bacterial metabolism and classification.
TestOxidase™ stands out as a fast, reliable, and user-friendly tool for microbiologists. Its ability to differentiate between oxidase-positive and oxidase-negative bacteria makes it indispensable in bacterial identification workflows.